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Establishment and Characterization of Human Neuroblastoma Cell Lines¹

Divisions of Experimental Pathology [H. R. S., K. H.], Oncology [J. M. G.], and Genetics [P. S. M.], Joseph Stokes, Jr. Research Institute of the Children's Hospital of Philadelphia and the Department of Pediatrics, The University of Pennsylvania, Philadelphia 19104, and the Institute for Cancer Research [H. M.], Philadelphia, Pennsylvania 19111

Three new tissue culture cell lines, CHP-100, CHP-126, and CHP-134, have been established from explant cultures of human neuroblastoma. The cell lines have been characterized with respect to morphology, chromosome constitution, growth, neural enzyme content, and their ability to grow in nude mice. The cells grow as dense masses comprised of fibroblast- or neuroblast-like cells with small processes. The cell lines differ in their neural enzyme activity. The chromosomal content of the 3 cell lines is near diploid, and all are capable of forming tumors in nude mice. The morphological findings indicate that the cells in culture resemble those found in the tumor, and the enzyme activities are consistent with those of nervous tissue. Thus, the morphological, biochemical, and tumorigenic properties confirm that the 3 cell lines are neoplastic cells of neural origin.

¹ This research was supported in part by National Cancer Institute Research Grants CA-1448903 and CA-13451-04 and in part by NIH Aging Program Project Grant AG-00378-04.

² To whom requests for reprints should be addressed, at Division of Experimental Pathology, Children's Hospital of Philadelphia, 34th Street and Civic Center Boulevard, Philadelphia, Pa. 19104.

³ Present address: Department of Dermatology, Hahnemann Medical College, Philadelphia, Pa.

Received 11/20/75. Accepted 5/26/76.