This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Buell, D. N. Articles by Isersky, C. Search for Related Content PubMed PubMed Citation Articles by Buell, D. N. Articles by Isersky, C.

Cell Cycle and Morphological Changes during Growth and Differentiation of a Rat Basophilic Leukemia Cell Line

Immunology Branch [D. N. B.] and Laboratory of Pathology [B. J. F.], National Cancer Institute, and Section on Chemical Immunology, Arthritis and Rheumatism Branch, National Institute of Arthritis, Metabolism and Digestive Diseases [H. M., C. I.], NIH, Bethesda, Maryland 20014

Cells of the rat basophilic leukemia cell line RBL-1 differentiate maximally when permitted to achieve growth arrest in a high-density stationary phase, in which the cell number is constant, and the cells are arrested in a G₁ phase of the cycle. Features of differentiation are the accumulation of large basophilic granules and increases in membrane receptors for immunoglobulin E. However, changes in histamine content did not parallel granule development or changes in immunoglobulin receptor concentration. During rapid "forced exponential" growth, the cell number doubles every 8 hr, 50% of the cells are in S phase, and differentiation is minimal.

¹ Present address: Division of Hematology and Oncology. Department of Medicine, Childrens Hospital Medical Center, Boston, Mass. 02115.

² To whom correspondence should be addressed.

Received 7/30/75. Accepted 5/25/76.