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A Cyclic Adenosine 3':5'-Monophosphate-mediated Effect of Cholera Toxin on High-Molecular-Weight Glycoprotein Species of Malignant Cells¹

Center of Microbiology and Cell Biology, Instituto Venezolano de Investigaciones Cientificas, Apartado 1827, Caracas, Venezuela

A comparison of the Pronase-sensitive glycosylated species detectable under permissive and nonpermissive conditions by normal rat kidney cells transformed by a temperature-sensitive derivative of Rous sarcoma virus reveals a relative decreased labeling of high-molecular-weight glycosylated species under conditions that allow the expression of transformation, in medium supplemented either with 0.5% calf serum or with human ₂-macroglobulin, 100 µg/ml. Exposure of the cultures to cholera toxin or dibutyryl cyclic adenosine 3':5'-monophosphate leads to a relative increase in the glycosylation of the high-molecular-weight species in both wild-type transformed and temperature-sensitive cells exposed to conditions that allow the expression of transformation.

¹ Supported in part by CONICIT (Venezuela) under Grant 31.26.S1-0469. This support is gratefully acknowledged.

Received 11/12/75. Accepted 6/ 7/76.