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The Effect of Adriamycin on Cell Cycle Progression and DNA Replication in Chinese Hamster Ovary Cells¹

Department of Physics, The University of Texas System Cancer Center, M. D. Anderson Hospital and Tumor Institute, Houston, Texas 77030

Chinese hamster ovary cells were treated with adriamycin (ADR) at various stages of the cell cycle in order to determine the effect on cell cycle progression and subsequent DNA replication. Concentrations of ADR up to 5 µg/ml for 15 min produced no progression delay in cells treated 1 hr prior to mitosis, and concentrations of 2 µg/ml or lower had no effect on the progression to mitosis of G₂ or late S-phase cells. However, cells treated in mid-S phase showed a significant, dose-dependent delay at concentrations as low as 0.5 µg/ml. This cell cycle effect on progression was also reflected in the rates of DNA replication in the subsequent S phase. DNA replication was not inhibited by 5 µg/ml for 30 min when applied in either the G₁, M, or G₂ phase. In contrast, when ADR was applied in the S or G₁ phases of the previous cell cycle, a marked depression in the rate of replication of DNA was observed. This was found to be the case for DNA synthesized both before and after the ADR treatment.

¹ This work was supported in part by National Cancer Institute Grant CA-04484 and Contract N01 CN 61156.

Received 3/12/76. Accepted 10/12/76.