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Polyamine-stimulated Growth of Cultured Rat Urinary Bladder Epithelial Cells¹

Veterans Administration Hospital and Department of Urology, University of Tennessee Center for the Health Sciences, Memphis, Tennessee 38104

A technique for isolating and establishing long-term cultures of rat urinary bladder epithelium has been devised. Cells isolated and cultured by this method have been grown for 12 weeks without subculturing. Rat bladder epithelial cells require the addition of putrescine, spermine, and spermidine to attain maximum growth and long-term survival. Monolayer cultures have been subcultured and carried through five passages.

¹ This work was supported by United States Veterans Administration Project 4323-01; by USPHS Research Grant CA-16765 from the National Cancer Institute through the National Bladder Cancer Project; and by Contract 641-42133(c), National Center for Toxicological Research, Department of Health, Education, and Welfare.

² To whom reprint requests should be addressed, at Veterans Administration Hospital, 1030 Jefferson Avenue, Memphis, Tenn. 38104.

Received 7/19/76. Accepted 10/18/76.

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