This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mayall, B. H. Articles by Rowley, J. D. Search for Related Content PubMed PubMed Citation Articles by Mayall, B. H. Articles by Rowley, J. D.

Quantification by DNA-based Cytophotometry of the 9q+/22q-Chromosomal Translocation Associated with Chronic Myelogenous Leukemia¹

Biomedical Sciences Division, Lawrence Livermore Laboratory, Livermore, California 94550 [B. H. M., A. V. C., D. H. M.], and Department of Medicine, University of Chicago and the Franklin McLean Memorial Research Institute, Chicago, Illinois 60637 [J. D. R.]

DNA-based cytophotometry was used to analyze metaphase chromosomes in four patients with chronic myelogenous leukemia. In three of these patients, both Philadelphia chromosome (Ph¹)-positive and Ph¹-negative cells were measured. On the basis of these three patients, the characteristic 9q+/22q- translocation of chronic myelogenous leukemia involves the net transfer of 0.325% of the autosomal genome; there is no evidence of net gain or loss of DNA (apart from duplication of the Ph¹ chromosome in one patient), and no significant difference is found in the amount of DNA transferred in different patients. Significant differences are found among patients in the derived Chromosomes 9 and the Ph¹ chromosomes and are ascribed to preexisting variations in the Ph¹-negative cells of these patients. There is no evidence in these patients of any further cytogenetic lesion associated with chronic myelogenous leukemia.

¹ This work was performed under the auspices of the United States Energy Research and Development Administration Contract W-7405-ENG-48 and was supported in part by USPHS Grant GM-20291.

² To whom requests for reprints should be addressed.

Received 4/ 7/77. Accepted 7/ 8/77.

This article has been cited by other articles:

				J. Rowley Identification of the constant chromosome regions involved in human hematologic malignant disease Science, May 14, 1982; 216(4547): 749 - 751. [Abstract] [PDF]