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[Cancer Research 37, 3639-3643, October 1, 1977]
© 1977 American Association for Cancer Research
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A Simplified Method for Production and Growth of Multicellular Tumor Spheroids

John M. Yuhas, Albert P. Li, Andrew O. Martinez and Aaron J. Ladman1

Cancer Research and Treatment Center [J. M. Y., A. P. L., and A. O. M.] and Departments of Radiology [J. M. Y., A. P. L.], and Anatomy [A. J. L.], University of New Mexico, Albuquerque, New Mexico 87131

A new technique, based on the growth of tumor cells in liquid media over an agar base, has been developed for the formation and growth of multicellular tumor spheroids. All of the 11 transformed cell lines tested formed multicellular tumor spheroids, while none of the 8 normal cell types tested did so. The advantages of the present technique over older methods include its simplicity, generality, and experimental flexibility.

1 Supported by the Division of Cancer Centers and Resources, National Cancer Institute, through Grant 1-P30-C-21074-01.

Received 4/11/77. Accepted 6/30/77.




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Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
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Copyright © 1977 by the American Association for Cancer Research.