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Cell Cycle Dependency of Metastatic Lung Colony Formation¹

Section of Experimental Radiotherapy, The University of Texas System Cancer Center, M. D. Anderson Hospital and Tumor Institute, Houston, Texas 77030

Exponentially growing FSA 1233 cells, one of the clones isolated from a mouse fibrosarcoma, were synchronized by fractionation according to cell size by centrifugal elutriation. Cells from each fraction were analyzed by flow microfluorometry to determine the stages of the cell cycle and were injected i.v. to determine lung colony-forming efficiency. In vitro plating efficiency of these cells was similar throughout the cell cycle except for a slight reduction at G₂ + M. On the other hand, lung colony-forming efficiency showed marked cell cycle and cell size dependencies, being lowest at G₁, highest at S, and declining slightly at G₂.

¹ This study was supported in part by the Department of Health, Education, and Welfare, NIH, National Cancer Institute Grants CA-11138, CA-06294, CA-18628, and CA-17364. Animals used in this study were maintained in facilities approved by the American Association for Accreditation of Laboratory Animal Care, and in accordance with current United States Department of Agriculture and Department of Health, Education, and Welfare, NIH, regulations and standards.

Received 4/11/77. Accepted 7/ 8/77.