This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bhuyan, B. K. Articles by Ogunbase, O. Search for Related Content PubMed PubMed Citation Articles by Bhuyan, B. K. Articles by Ogunbase, O.

Sensitivity of Different Cell Lines and of Different Phases in the Cell Cycle to Hyperthermia¹

The Upjohn Company, Kalamazoo, Michigan 49001

The sensitivity of different cell lines (Chinese hamster ovary, HeLa, L1210, and P388) to 43° was compared. Chinese hamster ovary and HeLa cells were much less temperature sensitive than were L1210 or P388 cells. This difference persisted even when HeLa and L1210 cells were grown in the same medium and suggested that this was an inherent difference between the cell lines.

Of all the cell lines, 7-day L1210 ascites, maintained by transfer in mice, were the most sensitive. The greater sensitivity of these cells, as compared to 4-day L1210 ascites or cells in culture, may be explained by the difference in their growth stages. The 7-day ascites cells would be in stationary growth as compared to the exponentially growing 4-day ascites or the cells in culture.

The temperature sensitivity of Chinese hamster ovary cells in different parts of the cell cycle was determined. Mid- and late-S-phase cells were more sensitive than cells in mitosis or early S; G₁ and G₂ cells were the least sensitive. In a partially synchronized culture, the heat sensitivity of L1210 cells increased with increased percentage of cells in S phase.

¹ Supported in part by Contract 1-CM-43753 with the Division of Cancer Treatment, NIH, Department of Health, Education, and Welfare.

² To whom requests for reprints should be addressed, at Cancer Research — 7252/25/4. The Upjohn Co., Kalamazoo, Mich. 49001.

Received 1/23/77. Accepted 7/20/77.

This article has been cited by other articles:

				Y. Daniely and J. A. Borowiec Formation of a Complex between Nucleolin and Replication Protein A after Cell Stress Prevents Initiation of DNA Replication J. Cell Biol., May 15, 2000; 149(4): 799 - 810. [Abstract] [Full Text] [PDF]