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Scanning Electron Microscopy of the Surfaces of Hamster Embryo Cells Transformed by Herpes Simplex Virus¹

Department of Microbiology and Specialized Cancer Research Center [R. G., R. F.], and Department of Anatomy [V. M., B. M.], The Milton S. Hershey Medical Center, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033

The surfaces of normal hamster embryo fibroblast (HEF) cells were examined by scanning electron microscopy. Surface characteristics of HEF cells were compared to those of cells derived from a primary tumor induced in hamsters following s.c. inoculation of herpes simplex virus type 1-transformed HEF cells (14-012-8-1) and to the surfaces of cells derived from a metastatic tumor to the lung induced by the same cells.

The most obvious difference in the surface characteristics of the examined cells was the morphology of the microvilli. In the few HEF cells that possessed microvilli, the distribution was uneven, and the lengths of the microvilli and the filopodia were variable. However, the surfaces of both tumor cell lines showed large numbers of microvilli which were evenly distributed over the surface of the cells, giving an almost "hairy" appearance. Long filopodia were occasionally observed on the surface of the primary tumor cell line and on the cell line derived from the metastatic tumor. Ruffles and blebs were occasionally observed on HEF cells and on the primary tumor cells but were not seen on the cells of the metastatic tumor.

¹ Supported by Contract NO1 CP 53516 within the Virus Cancer Program of the National Cancer Institute and Grant CA 18450 awarded by the National Cancer Institute, Department of Health, Education, and Welfare.

² Recipient of a Leukemia Society of America Scholar Award. To whom requests for reprints should be addressed.

Received 2/ 3/77. Accepted 9/ 8/77.