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Inhibition of DNA Polymerase from L1210 Murine Leukemia by a Sulfhydryl Reagent from Agaricus bisporus¹

Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710

The 490 quinone, a natural sulfhydryl-arylating reagent from the mushroom, Agaricus bisporus, markedly inhibited L1210 murine leukemia DNA polymerase while resulting in little inhibition of DNA polymerase ß from this source. This quinone was more strongly inhibitory than p-chloromercuribenzoate or N-ethylmaleimide and was less readily neutralized by sulfhydryl-containing molecules such as dithioerythritol. Preliminary experiments indicate that DNA protects DNA polymerase from inhibition by the 490 quinone. The inhibition of DNA synthesis by quinone 490 may contribute significantly to the cytotoxicity of this compound and to the potential of -L-glutaminyl-4-hydroxybenzene as an antitumor agent.

¹ This investigation was supported in part by Grant Ca19013, awarded by the National Cancer Institute, Department of Health, Education, and Welfare, and by USPHS Grant 3-TO1-GM00726.

² To whom requests for reprints should be addressed.

Received 7/18/76. Accepted 11/ 1/76.

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