This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ralph, P. Articles by Nakoinz, I. Search for Related Content PubMed PubMed Citation Articles by Ralph, P. Articles by Nakoinz, I.

Direct Toxic Effects of Immunopotentiators on Monocytic, Myelomonocytic, and Histiocytic or Macrophage Tumor Cells in Culture¹

Sloan-Kettering Institute for Cancer Research, Rye, New York 10580

Four murine monocyte, myelomonocyte, and histiocyte or macrophage tumor cell lines adapted to culture were growth inhibited by one or more of the following macrophage-activating substances: Mycobacterium bovis, Bacillus Calmette-Guérin strain, zymosan, lipopolysaccharide, and dextran sulfate, as well as tuberculin purified protein derivative, but not latex beads. Lipopolysaccharide was effective with one line at 4 ng/ml. All four lines actively phagocytosed zymosan and latex beads. In many cases the growth inhibition was apparently immediate but only cytostatic, and cell proliferation resumed upon removal of the drug. Bacillus Calmette-Guérin, live or boiled, was toxic to some of the tumor lines.

Synthesis of lysozyme by all the cell lines in the monocyte series and production of granulocyte colony-stimulating factor by the myelomonocytic leukemia were not inhibited during several days of zero growth conditions in the presence of drugs.

Since these agents had no direct effect on other hematopoietic tumor types (myeloma, T-lymphoma, mastocytoma) at the same or up to 10⁴ higher concentrations, it is proposed that the sensitive tumors retain specific receptors for immunostimulants, either at the cell surface or within the cell in the case of phagocytosable particles. The binding of these agents to physiological receptors leads to stimulation and mitogenesis in normal macrophages and lymphocytes but leads to growth inhibition without affecting differentiated functions in the corresponding tumor lines.

¹ Supported by Grants BMS 75-19734 from the National Science Foundation and AI-12741 and NCI-08748-10A from the NIH.

Received 7/14/76. Accepted 11/ 1/76.

This article has been cited by other articles:

				M. Capone, P. Romagnoli, F. Beermann, H. R. MacDonald, and J. P. M. van Meerwijk Dissociation of thymic positive and negative selection in transgenic mice expressing major histocompatibility complex class I molecules exclusively on thymic cortical epithelial cells Blood, March 1, 2001; 97(5): 1336 - 1342. [Abstract] [Full Text] [PDF]

				R. M. Rakita, N. N. Vanek, K. Jacques-Palaz, M. Mee, M. M. Mariscalco, G. M. Dunny, M. Snuggs, W. B. Van Winkle, and S. I. Simon Enterococcus faecalis Bearing Aggregation Substance Is Resistant to Killing by Human Neutrophils despite Phagocytosis and Neutrophil Activation Infect. Immun., November 1, 1999; 67(11): 6067 - 6075. [Abstract] [Full Text] [PDF]

				J. P.M. van Meerwijk and H. R. MacDonald In Vivo T-Lymphocyte Tolerance in the Absence of Thymic Clonal Deletion Mediated by Hematopoietic Cells Blood, June 1, 1999; 93(11): 3856 - 3862. [Abstract] [Full Text] [PDF]

				A. E. Frankel, M. Lilly, R. Kreitman, D. Hogge, M. Beran, M. H. Freedman, P. D. Emanuel, C. McLain, P. Hall, E. Tagge, et al. Diphtheria Toxin Fused to Granulocyte-Macrophage Colony-Stimulating Factor Is Toxic to Blasts From Patients With Juvenile Myelomonocytic Leukemia and Chronic Myelomonocytic Leukemia Blood, December 1, 1998; 92(11): 4279 - 4286. [Abstract] [Full Text] [PDF]

				J. Ravetch, A. Luster, R Weinshank, J Kochan, A Pavlovec, D. Portnoy, J Hulmes, Y. Pan, and J. Unkeless Structural heterogeneity and functional domains of murine immunoglobulin G Fc receptors Science, November 7, 1986; 234(4777): 718 - 725. [Abstract] [PDF]