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Culture of Cloned Cells from the Mouse Preputial Gland Tumor ESR 586¹

Departments of Environmental Medicine [T. G. R.], Cell Biology [L. P.], and Dermatology [V. R. W.], New York University Schools of Medicine, New York, New York 10016

Cells from the mouse preputial gland tumor ESR 586 have been cultured and cloned. Trypsin-ethylenediaminetetraacetate was used to obtain single cells. The cells are grown in a modified CMRL-1415 medium supplemented with 10% fetal calf serum. Clones tend to fall into two classes: Class 1, those that undergo morphological differentiation in liquid medium to form round bodies filled with lipid vacuoles; and Class 2, those that do not differentiate. Preliminary studies on the control of differentiation in Class 1 clones suggest that a minimum cell density is required before differentiation takes place. Analysis of the lipids from differentiating and nondifferentiating clones reveals the presence of sebaceous-type lipids in differentiating clones only. No requirement for testosterone was found for these cells.

¹ This research was supported in part by USPHS Grants CA-19421 and AM-18792, by Research Funds from the Department of Dermatology of this University, and it is also part of a Center Program Support by the National Institutes of Environmental Health Sciences, NIH, Grant ES-00260.

² To whom requests for reprints should be addressed.

Received 8/ 6/76. Accepted 11/ 9/76.