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Establishment of a Human Leiomyosarcoma Cell Line¹

Departments of Obstetrics and Gynecology [I. I., S. N., S. N., S. K.] and Pathology [A. M.], School of Medicine, Keio University, 35, Shinanomachi, Shinjuku-ku, Tokyo 160, and Department of Virology and Rickettsiology, National Institute of Health, Kamiosaki, Shinagawa-ku, Tokyo 141, Japan [H. O.]

A cell line designated SKN was established from the human uterine leiomyosarcoma of a 52-year-old female. The cell line has grown well and the serial passages were successively carried out 82 times within 12 months. The monolayer cultured cells revealed anaplastic and pleomorphic features, and they multipled rapidly without contact inhibition. Electron microscope studies revealed myoibrils but no virus-like particles, while chromosomal studies showed that all cultured cells were hyperploid, the modal number was 112, and the marker chromosome was present. The cells were transplanted into an immune-depressed hamster cheek pouch and produced a histological leiomyosarcoma resembling the original tumor.

¹ This work was supported by a Grant from Cancer Research from the Japanese Ministry of Education.

Received 7/28/76. Accepted 11/16/76.

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