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Characterization of a New Continuous Cell Line Derived from a Human Retinoblastoma¹

Wills Eye Research Institute, Philadelphia, Pennsylvania 19130 (R. M., T. W. S.), and Children's Hospital, Philadelphia, Pennsylvania 19104 (M. M.)

A new continuous cell line derived from a human retino blastoma has been established. This cell line, WERI-Rb1, has been maintained in vitro since December 1974. The purpose of this investigation was to characterize WERI-Rb1 on the basis of morphology, growth, tumorigenicity, cytogenetics, and to compare this cell line with Y79, a human retinoblastoma cell line established at another institution. Morphologically, both cell lines were similar; each spontaneously grew as a suspension of small round cells in grapelike clusters. Each exhibited growth of cells in rosettes, as well as unusual chain formations. Growth rates differed: the population-doubling times for WERI-Rb1 and Y79 were 96 and 33 hr, respectively. When the negative surface charge on a plastic tissue culture flask was changed, each cell line grew as a monolayer. Y79 could be cloned in soft agar; WERI-Rb1 could not. An inoculum of 10⁷ WERI-Rb1 or Y79 cells produced a retinoblastoma in test rabbits. Karyological examination showed each cell line to have a stable, near diploid chromosome number. Although large markers were observed in each cell line, they shared no common marker.

¹ This investigation was supported by National Cancer Institute Grant CA 12850-04 and General Research Support Grant RR 05510. Presented in part at the Association for Research in Vision and Ophthalmology Meeting, Sarasota, Fla., April 1976 and at the Twenty-Seventh Annual Meeting of the Tissue Culture Association, Philadelphia, Pa., June 1976 (14).

² To whom requests for reprints should be addressed.

Received 9/ 3/76. Accepted 12/28/76.

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