This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bresnick, E. Articles by Conney, A. H. Search for Related Content PubMed PubMed Citation Articles by Bresnick, E. Articles by Conney, A. H.

Epidermal Hyperplasia after Topical Application of Benzo(a)pyrene, Benzo(a)pyrene Diol Epoxides, and Other Metabolites¹

Department of Biochemistry, The University of Vermont School of Medicine, Burlington, Vermont 05401 [E. B.]; Department of Anatomy, Medical College of Georgia, Augusta, Georgia 30902 [T. F. M.]; National Institute of Arthritis, Metabolism and Digestive Diseases, NIH, Bethesda, Maryland 20014 [H. Y., D. M. J.]; and Department of Biochemistry and Drug Metabolism, Hoffmann-La Roche Inc., Nutley, New Jersey 07110 [W. L., A. W. W., A. H. C.]

The effects of benzo(a)pyrene (BP) and 22 derivatives upon the number of nuclei per unit length of epidermis, the number of cell layers of epidermis, and the thickness of the epidermal layer were studied. Several derivatives of BP induced changes in epidermal morphology that are typical of those produced by various agents that promote skin tumorigenesis after application of an initiator. The most potent compounds tested were the BP diol epoxides, (±)-7ß,8-dihydroxy-9ß,10ß-epoxy-7,8,9,10-tetrahydrobenzo-(a)pyrene (diol epoxide 1) and (±)-7ß,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene (diol epoxide 2). These derivatives were followed in activity by 9-hydroxybenzo(a)pyrene, 2-hydroxybenzo(a)pyrene, and by 9,10-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene. The possible implications of these results with regard to the carcinogenicity of BP on mouse skin are discussed.

¹ This work was supported in part by Grant CA 17565 from the National Cancer Institute.

² To whom requests for reprints should be addressed.

Received 10/ 6/76. Accepted 12/27/76.

This article has been cited by other articles:

				W. Ouyang, Y. Hu, J. Li, M. Ding, Y. Lu, D. Zhang, Y. Yan, L. Song, Q. Qu, D. Desai, et al. Direct evidence for the critical role of NFAT3 in benzo[a]pyrene diol-epoxide-induced cell transformation through mediation of inflammatory cytokine TNF induction in mouse epidermal Cl41 cells Carcinogenesis, October 1, 2007; 28(10): 2218 - 2226. [Abstract] [Full Text] [PDF]

				C. Huang, Y. Huang, J. Li, W. Hu, R. Aziz, M.-s. Tang, N. Sun, J. Cassady, and G. D. Stoner Inhibition of Benzo(a)pyrene Diol-Epoxide-induced Transactivation of Activated Protein 1 and Nuclear Factor {kappa}B by Black Raspberry Extracts Cancer Res., December 1, 2002; 62(23): 6857 - 6863. [Abstract] [Full Text] [PDF]

				H. Rubin Synergistic mechanisms in carcinogenesis by polycyclic aromatic hydrocarbons and by tobacco smoke: a bio-historical perspective with updates Carcinogenesis, December 1, 2001; 22(12): 1903 - 1930. [Abstract] [Full Text] [PDF]

				C. C. Özbal, P. L. Skipper, M. C. Yu, S. J. London, R. R. Dasari, and S. R. Tannenbaum Quantification of (7S,8R)-Dihydroxy-(9R,10S)-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene Adducts in Human Serum Albumin by Laser-induced Fluorescence: Implications for the in Vivo Metabolism of Benzo[a]pyrene Cancer Epidemiol. Biomarkers Prev., July 1, 2000; 9(7): 733 - 739. [Abstract] [Full Text]