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Comparison of Nuclear Nonhistone Phosphoproteins of Rat Liver and Novikoff Hepatoma¹

Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030

As part of a continuing comparison of nuclear proteins of tumors and other tissues, ³²P-labeled nuclear proteins were extracted successively with 0.15 and 0.35 M NaCl from the nuclei of normal, regenerating, and thioacetamide-treated rat liver as well as Novikoff hepatoma 3 hr after injection of ³²P₁ into rats. Separation of proteins of these fractions with aqueous phenol was carried out before two-dimensional electrophoresis on polyacrylamide gels. By autoradiography many common spots were found, but four ³²P-labeled protein spots, CU', C13p, C21p, and CMp, were found in the Novikoff hepatoma and not in the various liver samples studied. Two spots, B6 and B10, were found in the liver patterns and not in the tumor. Spot B33 was very dense in regenerating liver but was only a faint spot in thioacetamide-treated liver. The greater density of Spots CU', C13p, C21p, and CMp in the tumor patterns is consistent with the increased density reported earlier for spots of the C-region of a variety of tumors.

¹ These studies were supported by the Cancer Research Center Grant CA-10893, P.1 awarded by the National Cancer Institute, Department of Health, Education and Welfare, the Pauline Sterne Wolff Memorial Foundation, a gift from Dr. and Mrs. O. A. Breiling, and a generous gift from Mr. Jack Hutchins.

Received 9/14/76. Accepted 1/26/77.