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Procedures for Radioimmunoassay of the Mouse Mammary Tumor Virus¹

Institute for Medical Research, Camden, New Jersey 08103

A procedure for radioimmunoassay of the major glycoprotein antigen derived from murine mammary tumor virus is described. The assay is sensitive to 0.05 ng of antigen and is highly reproducible. The antigen, gp55, has been found to be group specific and will detect viruses in 13 separate mouse strains, as well as from continuous cell lines. Factors affecting the assay have been examined.

¹ This work was supported by Contract N01-CP-53561 within The Virus Cancer Program and Grant CA-08740 from the National Cancer Institute.

² Present address: Hahnemann Medical College, 230 N. Broad St., Philadelphia, Pa. 19102, where requests for reprints can be sent.

Received 11/19/76. Accepted 2/16/77.