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Immunofluorescent Characterization of Rat Kidney Tumors According to the Distribution of Actin as Revealed by Specific Antiactin Antibody¹

Baker Medical Research Institute [G. C. H.], and Department of Pathology and Immunology, Monash University Medical School, Commercial Road, Prahran, Victoria 3181, Australia [B. H. T.]

A series of 15 mesenchymal and 10 cortical epithelial tumors induced in the rat kidney by dimethylnitrosamine was investigated for immunofluorescent reactivity with a human antiactin antibody. Cells of epithelial tumors showed staining restricted to peripheral sites, corresponding to the brush border region. All various neoplastic cell forms comprising renal mesenchymal tumor were characterized by cytoplasmic staining in patterns that varied with cell type. Epithelial profiles in the form of tubules and islands of epithelium showed staining patterns, or absence of them, consistent with their identity as sequestered segments of preexisting nephrons. It is suggested that the difference in actin distribution within the cytoplasm of cells of the two types of renal neoplasm, mesenchymal and epithelial, might reflect their difference in local invasive growth.

¹ This research was supported by the A. A. Thomas Fellowship of the Anti-Cancer Council of Victoria and grants from the Anti-Cancer Council and the National Health and Medical Research Council.

² Arthur A. Thomas Fellow. Present address: Department of Pathology, University of Melbourne Medical Centre, Grattan Street, parkville, Victoria 3052, Australia. To whom requests for reprints should be addressed.

Received 12/30/76. Accepted 3/ 1/77.