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Selective Growth of Transformed Cell Lines by Rat Liver Perfusate¹

Departments of Medicine [M. F. S., J. D., A. L.], Microbiology [J. G., R. G., A. L.], and Physiology [L. S. J.]; and Specialized Cancer Research Center [A. L.], The Milton S. Hershey Medical Center, The Pennsylvania State University, Hershey, Pennsylvania 17033

Perfused rat liver releases growth-promoting activity for viral, spontaneous, and chemically transformed cells. After 5 days of incubation with perfusate, cell lines 3T12-NY (a spontaneous fibroblast transformant), NQ-T-1 (a chemically transformed fibroblast line), W-8 (a chemically transformed epithelial rat liver cell line), and H-50 (an SV40-transformed hamster fibroblast line) all exhibit significant increase in cell growth above controls. Their respective normal counterparts: 3T3 CI 42, A31-714, K-16, and HEF are not so stimulated. Within another set, the virally transformed mouse fibroblast cell line, SV3T3, exhibits a 27-fold increase in growth; however, 3T3 (mouse fibroblasts), P_y3T3 (polyomatransformed 3T3 cells), SV-Fl₂-101 (a flat revertant line), and SV-P_y-3T3 (a doubly transformed line) are nonresponsive.

Perfused rat liver also releases survival activity for SV-3T3 cells. The growth-stimulating activity in liver perfusate is selective for transformed cells. It is suggested that the liver may play a role in supporting neoplasia in vivo.

¹ This investigation was supported by USPHS Research Grants CA-15858, AM-13499, and AM-15658.

² To whom requests for reprints should be addressed.

Received 9/29/76. Accepted 3/ 1/77.