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DNA Transfection of Ecotropic Murine Leukemia Viruses in Mouse Cell Cultures¹

Cancer and Toxicology Program, Biology Division, Oak Ridge National Laboratory, and University of Tennessee-Oak Ridge Graduate School of Biomedical Sciences, Oak Ridge, Tennessee 37830

DNA's were isolated from cells chronically infected with N-, B-, or NB-tropic murine leukemia viruses and tested for infectious activity in various mouse cell cultures. Early detection of the DNA transfection is facilitated by growing the DNA-recipient cells in medium containing 10^-6 M hydrocortisone. Appropriate shearing of the DNA preparations may increase the efficiency of the transfection. With these procedures virus production of the transfected cells can be detected by XC plaque assay as early as 4 days after DNA inoculation in NIH 3T3 cells. Susceptibility of the mouse cell cultures to DNA transfection does not parallel their susceptibility to virion infection. Progeny viruses derived from the transfection show the same N- or B-tropic host range property as do the parent viruses.

¹ Research supported jointly by the Virus Cancer Program of the National Cancer Institute (NOI CP 6-0500) and the Energy Research and Development Administration under contract with Union Carbide Corporation and in part by the American Cancer Society.

² Postdoctoral fellow supported by Training Grant CA 05296. Present address: Experimental Pathology Branch, National Cancer Institute, NIH, Bethesda, Md. 20014.

³ To whom requests for reprints should be addressed, at Cancer and Toxicology Program, Biology Division, Oak Ridge National Laboratory, Post Office Box Y, Oak Ridge, Tenn. 37830.

Received 12/ 6/76. Accepted 3/ 1/77.