This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Bases, R. Articles by Neubort, S. Search for Related Content PubMed PubMed Citation Articles by Bases, R. Articles by Neubort, S.

Effects of Lucanthone on the Sedimentation Properties of DNA from HeLa Cells¹

Department of Radiology, Albert Einstein College of Medicine, Bronx, New York 10461

Exposure of HeLa cells to lucanthone (3 µg/ml) caused dissociation of a fast-sedimenting duplex DNA complex, as judged by lysis and sedimentation in alkaline sucrose gradients. The effect of lucanthone on the DNA complex resembled that of actinomycin D and ionizing radiation. Protein synthesis inhibitors such as cycloheximide or inhibitors of DNA synthesis such as hydroxyurea did not lead to dissociation of the complex. Lucanthone was more active than were hycanthone and five other closely related thiaxanthenones tested. Lucanthone promoted X-ray-induced denaturation of DNA in intact cells, as judged by their nuclear immunoreactivity to antinucleoside antibodies. Lucanthone did not inhibit repair of X-ray-induced DNA single-strand breaks.

¹ Supported in part by American Cancer Society, Inc., Grants PDT-20B and PDT-29A; National Cancer Institute Grant CA-20879; and the Mildred Werner League for Cancer Research, Great Neck, N. Y.

² A Jane and Arnold Ginsburg Fellow in Radiology. To whom requests for reprints should be addressed, at Department of Radiology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, N. Y. 10461.

Received 7/19/76. Accepted 4/ 7/77.