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Cell Surface Markers on Epithelial-Burkitt Hybrid Cells Superinfected with Epstein-Barr Virus¹

Department of Microbiology and Specialized Cancer Research Center, The Milton S. Hershey Medical Center, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033 [R. G.]; International Agency for Research on Cancer, 150 Cours Albert Thomas, 69008 Lyon, France [G. L., D. G.]; and Department of Molecular Immunology, Scripps Clinic and Research Foundation, La Jolla, California 92037 [S. F., M. A. P.]

Attempts were made to superinfect two epithelial-Burkitt hybrid cell lines, designated D98/HR-1 and D98/Raji, with Epstein-Barr virus (EBV) and to investigate the expression of some cell surface markers, including histocompatibility antigens, and the presence of B-cell markers, such as receptors for the third complement component and for monkey red blood cells. Successful superinfection of D98/HR-1 cells with EBV was made evident by the expression of early antigen and, to a lesser extent, virus capsid antigen. Only a rare D98/Raji cell was found to be positive for early antigen. The histocompatibility antigens of the parental cell lines D98, HR-1, and Raji were expressed on the surfaces of the hybrid cells. Receptors for third complement components b and d were not detected on the hybrid cells or on the D98 or HR-1 cell lines; they were found, however, on the Raji cells, indicating that EBV receptors and complement receptors can be separated. The significance of the infection of the hybrid cells with EBV and the expression of cell surface markers is described.

¹ This study was supported by Contracts N01 CO43296 and N01 CP53516 within the Virus Cancer Program of the National Cancer Institute, NIH, USPHS, and by Grants 1 P30 CA18450, AI13154, CA15038, CA16071, and CA16069. Part of this study was performed at the International Agency for Research on Cancer, Lyon, France, under the sponsorship of the Fogarty International Center, the NIH, and the Institut National de la Santé et de la Recherche Médicale, Paris, France. This is Publication 1192 from Scripps Clinic and Research Foundation.

² Leukemia Society of America Scholar. To whom requests for reprints should be addressed, at Department of Microbiology, The Milton S. Hershey Medical Center, The Pennsylvania State University, Hershey, Pa. 17033.

³ Recipient of an American Heart Established Investigatorship.

Received 1/26/77. Accepted 4/15/77.