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Metabolism of Dimethylnitrosamine and 1,2-Dimethylhydrazine in Cultured Human Bronchi

Human Tissue Studies Section, Experimental Pathology Branch, Carcinogenesis Program, Division of Cancer Cause and Prevention, National Cancer Institute, Bethesda, 20014 [C. C. H., H. A., G. D. S.]; Department of Pathology, University of Maryland School of Medicine, Baltimore, Maryland 21201 [E. M. M., B. F. T.]; and Department of Surgery, Washington Veterans Administration Hospital, Washington, D. C. 20420 [P. W. S.]

The metabolic activation of several chemical classes of procarcinogens is being studied in cultured human bronchi. Previous studies have shown that carcinogenic polynuclear aromatic hydrocarbons are metabolically activated by the bronchial epithelium. In the study reported here, dimethylnitrosamine and 1,2-dimethylhydrazine were also found to bind to both cellular DNA and protein. Bronchial DNA was methylated in both the O-6 and N-7 positions of guanine. In addition to polynuclear aromatic hydrocarbons, an aliphatic nitrosamine and a methylhydrazine can now be added to the list of xenobiotic chemical carcinogens metabolized by human bronchus.

¹ To whom requests for reprints should be addressed, at Building 37, Room 3A07, National Cancer Institute, NIH, Bethesda, Md. 20014.

² Supported in part by Contract NO1 CP 43237.

³ Supported in part by Interagency Agreement YO1 CP 60204.

Received 11/23/76. Accepted 4/29/77.