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Galactosyltransferase and Phosphatase Activities in Physiological Fluids from BALB/c Mice Bearing the YC8 Lymphoma¹

Institut d'Immuno Biologie, Hópital Broussais, 96 rue Didot, 75674 Paris Cedex 14, France

Physiological fluids from adult BALB/c mice were collected weekly or daily after the i.p. injection of YC8 tumor cells.

Galactosyltransferase activity on endogenous acceptors was enhanced from 1% in normal sera to 14.6% in sera collected on Day 11. Afterward, it decreased to 9.6%. In peritoneal fluids, galactosyltransferase activity was enhanced from 0 to 45.3% on Day 16. In urines, no significant activity was detected. Galactose transfer was enhanced in sera and peritoneal fluids until Day 10, in the presence of urines. Then, urines had an inhibitory effect. Variations of optimal pH and apparent K_m for uridine diphosphate galactose were observed among fluids collected from normal and ascitic mice.

Phosphatase activity was 85.6% in normal sera, decreased until Day 10 (11.2%), and was enhanced once more (21.4%) on Day 13. In peritoneal fluids, phosphatase activity was low, except on Days 5 (12.4%) and 9 (11%). In urines, no activity was detected, except on Day 6 (5.6%). Urines had an inhibitory effect on sera and an activator effect on peritoneal fluids.

A decrease of phosphatase activity and an enhancement of galactose transfer were related to tumor growth, as well as to the appearance of effectors and/or acceptors in urines.

¹ This work was supported by Institut de la Santé et de la Recherche Medicale, Unité 20, and by Centre National de la Recherche Scientifique, Laboratoire Associé No. 143.

² To whom requests for reprints should be addressed.

Received 12/15/76. Accepted 5/ 6/77.