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Gonadotropin Receptors in Experimentally Induced Ovarian Tumors in Mice¹

Departments of Medicine [S. K., J. R.] and Pathology [R. I. D.], New York University Medical Canter, New York, New York 10016

Gonadotropic hormones are required for the induction and maintenance of tumors arising in ovaries that have been transplanted to the spleens of gonadectomized mice. The characteristics of gonadotropin receptors for human chorionic gonadotropin (HCG)-luteinizing hormone on cells from these tumors of varying size, age, and morphology have been determined. The specific binding of ¹²⁵I-labeled HCG to cells obtained by collagenase digestion, 15 to 65 weeks postimplantation from granulosa cell or luteinized cell, or mixed granulosa-luteal tumors was analyzed by Scatchard plot. Neither the size, weight, duration of implantation, nor histological morphology affected the receptorbinding affinity [equilibrium dissociation constant (K_d), 6 x 10^-10 M], and, presumably, the receptor is qualitatively similar. In contrast, the number of HCG receptors per cell increased 17-fold and was related to the degree of morphological luteinization of the tumor. HCG-sensitive adenyl cyclase was also demonstrated and compared to HCG binding in a highly luteinized tumor.

¹ Supported by Grant PDT-6 from the American Cancer Society.

² To whom requests for reprints should be addressed.

Received 2/ 9/77. Accepted 5/ 6/77.