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Immunological Assay of Double-Helical Segments in RNA Fractions of Different Molecular Size Extracted from Acute Myeloid Leukemia Blast Cells¹

Laboratory of Molecular Hematology, Institute of Medical Pathology, University of Modena, 41100 Modena, Italy

Whole-cell RNA, extracted from acute myeloid leukemia blast cells, was fractionated by sedimentation through sucrose gradients. The proportion of double-helical segments present in each fraction was then determined by a quantitative microcomplement fixation assay that specifically measures double-helical RNA. Sizable amounts of double-helical segments were detected in all fractions of cellular RNA corresponding to S values higher than approximately 20. In all cell populations examined, the highest proportion of double-helical segments was found in RNA fractions sedimenting faster then the 45 S ribosomal precursor RNA, i.e., in fractions including only heterogeneous nuclear RNA.

¹ This research was supported by a grant from the Consiglio Nazionale delle Ricerche.

² To whom requests for reprints should be addressed, at the Institute of Medical Pathology, University of Modena, Policlinico, Modena 41100, Italy.

Received 9/ 7/76. Accepted 3/11/77.