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Differential Expression of Relevant Rous Sarcoma-associated Antigens in Cultured Cells¹

Lady Davis Institute for Medical Research, Jewish General Hospital, Montreal, Quebec [M. A. W., E. I., E. S.-L., M. Y.]; and Département de Microbiologie et d'Immunologie, Université de Montréal, Montréal, Québec, Canada [M. A. W.]

These studies compared the abilities of each of normal chicken embryo fibroblast (CEF) cells, Rous sarcoma virustransformed CEF cells, and Rous sarcoma (RS) tumor cells derived from the wings of chickens with growing neoplasms, as well as extracts and supernatant fluids of each of these cell types, to interact with the lymphocytes of chickens bearing tumors induced by Rous sarcoma virus. Cultured RS cells were more susceptible in ⁵¹Cr-release cytotoxicity assays to killing by the splenic lymphocytes of RS-bearing chickens than were Rous sarcoma virus-transformed CEF cells which, in turn, were more susceptible than normal CEF cells. In contrast, extracts (3 M KCl derived) of both RS cells and transformed CEF cells were equally capable of eliciting blastogenic responsiveness of circulating lymphocytes obtained from birds with tumors, while, in the case of supernatant fluids, significant reactivity was detected only when material derived from cultures of transformed CEF cells was used. Similar levels of responsiveness against such culture supernatant fluids were observed when circulating lymphocytes of chickens infected with avian leukosis viruses were studied. Lymphocytes of normal chickens served as control. These results point to a different expression of relevant detectable tumor-associated antigens in these various cell types. Scanning electron microscopy revealed few morphological differences between RS cells and transformed CEF cells, although both displayed considerably greater numbers of surface ruffles and microvilli than did normal CEF cells.

¹ This work was supported by research grants from both the National Cancer Institute of Canada and the Conseil de la Recherche en Sante du Quebec.

² Chercheur-boursier of the Conseil de la Recherche en Santé du Quebec.

Received 12/ 7/76. Accepted 5/18/77.