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A New in Vitro Cell Line Established from Human Oat Cell Carcinoma of the Lung¹

Division of Physiology and Pathology, National Institute of Radiological Sciences, Anagawa 4-9-1, Chiba-shi, Chiba, Japan 280 [H. O.], and The Institute of Pulmonary Cancer Research, School of Medicine, Chiba University, Inohana 1-8-1, Chiba-shi, Chiba, 280 [T. O.] Japan

A new tissue culture cell line, OAT-1975, has been established from explant cultures of human oat cell carcinoma of the lung, characterized and maintained for more than 1 year. The cells grew in a monolayered sheet with 24 hr of population-doubling time and are capable of clonal growth with about 60% plating efficiency. The cells still retain unique morphological and structural characteristics of oat cell carcinoma and showed "tumor takes" when transplanted into nude mice and conditioned young Syrian Golden hamsters. The chromosome constitution of the cells is hypertriploid with a modal peak at 74. The permanent growth integrity of the present cell line made it possible to utilize it for cell biological studies. Cell inactivation experiments indicated that the cells are sensitive to X-rays and Mitomycin C when compared to the responses of HeLa S3 cells.

¹ This work was supported by grants from the Japanese Ministry of Health and Welfare and the Japanese Ministry of Education for Cancer Research.

² To whom requests for reprints should be addressed.

Received 11/ 3/76. Accepted 6/ 8/77.

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