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A Radioimmunoassay for 1-ß-D-Arabinofuranosyluracil with Reference to Cross-Reactivity of 1-ß-D-Arabinofuranosylcytosine with an Antibody¹

Shionogi Research Laboratory, Fukushima-ku, Osaka, Japan 553 [T. O., S. M.], and The Institute of Molecular Biology, Syntex Research, Palo Alto, California 94304 [D. B. R., J. G. M.]

Antibodies directed against 1-ß-D-arabinofuranosyluracil have been produced in rabbits by immunization with a conjugate of 1-(5-O-succinyl-ß-D-arabinofuranosyl)uracil with human serum albumin. Two of four antibodies so obtained showed high specificity for 1-ß-D-arabinofuranosyluracil and allowed the development of a sensitive and reliable radioimmunoassay for this substrate. On the other hand, one antibody had a high affinity for 1-ß-D-arabinofuranosylcytosine. The binding of 1-ß-D-arabinofuranosylcytosine to this antibody was practically constant between pH 5.2 and 9.0, whereas 1-ß-D-arabinofuranosyluracil binding was affected drastically by pH. The pH-binding profile for 1-ß-D-arabinofuranosylcytosine and 1-ß-D-arabinofuranosyluracil was reminiscent of the specificity of ara-C-specific antibodies, which we previously obtained after immunization of rabbits with 1-(5-O-succinyl-ß-D-arabinofuranosyl)cytosine as a hapten.

¹ Contribution 135 from the Institute of Molecular Biology.

² To whom requests for reprints should be addressed.

Received 3/14/77. Accepted 6/13/77.