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Turnover of Cellular Carbohydrates in Normal and Rous Sarcoma Virus-transformed Cells¹

Department of Microbiology [J.G.L., A.H.H., M.J.W.], and Biochemistry [D.E.R., H.E.C.], University of Illinois, Urbana, Illinois 61801

We have analyzed the distribution of glucosamine-labeled polymers on the cell surface, in the growth medium, and inside the cell and the net turnover of these polymers during the process of malignant transformation of chick embryo fibroblasts by Rous sarcoma virus. The distribution of label and the turnover kinetics for hyaluronic acid, total glycoproteins, and chondroltins were found to be identical in both normal and transforming cultures. We conclude that nonspecific degradative processes are probably not involved in causing transformation-related alterations in cell surface carbohydrates, although degradation of specific macromolecules is not excluded.

¹ This work was supported by USPHS Grants CA 12467 and HD 8057 and by American Cancer Society Grant VC 205A.

² Present address: Department of Biochemistry, University of Illinois, Urbana, Ill. 61801.

³ Present address: Center for Cancer Biology, Massachusetts Institute of Technology, Cambridge, Mass. 02139.

⁴ Present address: Department of Biochemistry, School of Medicine, University of Pittsburgh, Pittsburgh, Pa. 15261.

⁵ Recipient of NIH Research Career Development Award CA 00092. To whom inquiries and requests for reprints should be addressed.

Received 2/17/77. Accepted 10/12/77.

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