This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Rogan, K. M. Articles by Humphreys, R. E. Search for Related Content PubMed PubMed Citation Articles by Rogan, K. M. Articles by Humphreys, R. E.

Heterogeneity in the Membrane Proteins of Human Lymphoid Cell Lines as Seen in Sodium Dodecyl Sulfate-Polyacrylamide Electrophoresis Slab Gels¹

Department of Pharmacology and Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605

The proteins of [³⁵S]methionine-labeled membranes of six human lymphoid cell lines were examined by electrophoresis in sodium dodecyl sulfate-polyacrylamide gradient slab gels in order to identify molecular differences among these tumors. The lymphoid cells were internally labeled with [³⁵S]methionine, their membranes were isolated, and the reduced and alkylated membrane proteins were treated electrophoretically in sodium dodecyl sulfate-polyacrylamide gradient slab gels. The gel patterns of over 100 membrane proteins per cell were highly complex but reproducible and, in that sense, constituted fingerprints of the individual tumors. Several proteins occurred uniquely on one or a few tumors. Some protein bands were identified to be serologically recognized membrane antigens by electrophoresis of immunopurified antigen in parallel to membrane samples. p44,12, a complex of proteins with molecular weights of 44,000 and 12,000 (HLA-A and -B antigens and ß₂-microglobulin), and p29,34, (HLA-D antigen) were identified in this manner. High-resolution sodium dodecyl sulfate-polyacrylamide gradient gel electrophoresis can be used to catalog and describe lymphocyte membrane proteins and perhaps to identify subsets of lymphoid cancers.

¹ This work was supported by Research Contract N01-CB-64072 from the National Cancer Institute.

² Present address: Tufts University School of Medicine, 200 Harrison Ave., Boston, Mass. 02111.

³ Recipient of a Cancer Research Scholar Award from the American Cancer Society, Massachusetts Division, Inc. To whom requests for reprints should be addressed.

Received 3/12/78. Accepted 7/27/78.