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Effect of Adriamycin and Analogs on the Nuclear Fluorescence of Propidium Iodide-stained Cells¹

Comprehensive Cancer Center for the State of Florida, University of Miami School of Medicine, Miami, Florida 33152 [A. K., R. N. G.], and Sidney Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115 [M. I.]

Adriamycin (ADR) and N-trifluoroacetyladriamycin-14-valerate, respectively, inhibit and enhance the nuclear fluorescence of cells stained with propidium iodide for DNA per cell estimation by flow cytometry. In cells incubated with ADR, the reduction in fluorescence is gradually manifested due to the slow intracellular drug transport. In contrast the effect of N-trifluoroacetyladriamycin-14-valerate on propidium iodide nuclear fluorescence is seen within 5 min of incubation. The effect of ADR on propidium iodide nuclear fluorescence could be detected in vivo even after 24 hr of ADR administration.

¹ These studies were supported by Grants CA-23688 and CA-19118 from the National Cancer Institute, United States Department of Health, Education and Welfare. A preliminary report of this work was first presented at the 69th Annual Meeting of the American Association for Cancer Research, Washington, D. C., 1978 (10). This work was started at Sidney Farber Cancer Institute, Boston and continued at University of Miami Medical School.

Received 7/11/77. Accepted 7/27/78.