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Increased Tumor Immunity in Mice Inoculated with Muconomycin A-treated B16 Melanoma Cells¹

Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire 03755

B16 melanoma cells were treated in vitro with muconomycin A, a long-lasting inhibitor of protein and glycoprotein synthesis, to reduce cellular sialic acid. Two i.p. inoculations of 10⁷ muconomycin-treated cells into female C57BL/6 mice, followed by challenge with homologous live cells, resulted in a significant decrease in tumor incidence when compared to the results of inoculation with untreated cells (p < 0.01). Inoculation of mice with cells treated with neuraminidase resulted in little or no decrease in tumor incidence. Effective immunity was dependent on the number of cells injected and was found only with the i.p. route of inoculation into female mice.

¹ Supported by USPHS Grants 1RO1-CA 15085 and 1RO1-CA17293 from the National Cancer Institute and by Grant E557 from the American Cancer Society.

² Recipient of USPHS Postdoctoral Fellowship 1-FO2-CA55546-01 from National Cancer Institute, and a postdoctoral fellowship from the Leukemia Society of America. Present address: Department of Medicien, Dartmouth Medical School, Hanover, N.H. 03755. To whom requests for reprints should be addressed.

Received 2/14/78. Accepted 8/ 7/78.