This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cha, Y.-N. Articles by Bueding, E. Search for Related Content PubMed PubMed Citation Articles by Cha, Y.-N. Articles by Bueding, E.

Enhancement of Liver Microsome Epoxide Hydratase Activity in Rodents by Treatment with 2(3)-tert-Butyl-4-hydroxyanisole¹

Departments of Pathobiology [Y-N. C., E. B.] and Pharmacology and Experimental Therapeutics [F. M., E. B.], Johns Hopkins Medical Institutions, Baltimore, Maryland 21205

Administration of the antioxidant 2(3)-tert-butyl-4-hydroxyanisole (BHA) in the diet caused a marked increase in the specific activity of epoxide hydratase (EC 4.2.1.63) in hepatic microsomes of CD-1 mice. The increases in epoxide hydratase activities produced by BHA were far greater (11-fold) than were those produced by the administration of well-known enzyme inducers such as 3-methylcholanthrene, phenobarbital, and Aroclor 1254 (2- to 3-fold). The near-maximal increase in epoxide hydratase activity was observed after feeding of the BHA diet for 3 days. When BHA was administered by gastric intubation, the level of increase was only 75% of that attained by feeding BHA in the diet. The increase in epoxide hydratase activity produced by BHA treatment of Sprague-Dawley rats was not as pronounced (<3-fold) as that observed in CD-1 mice.

¹ Supported by grants and contracts from the NIH (GM 16492 and CA 18251). A preliminary account of these findings has been presented (5).

Received 4/26/78. Accepted 8/23/78.

This article has been cited by other articles:

				A. H. Conney Enzyme Induction and Dietary Chemicals as Approaches to Cancer Chemoprevention: The Seventh DeWitt S. Goodman Lecture Cancer Res., November 1, 2003; 63(21): 7005 - 7031. [Abstract] [Full Text] [PDF]

				T. Ishii, K. Itoh, J. Akasaka, T. Yanagawa, S. Takahashi, H. Yoshida, S. Bannai, and M. Yamamoto Induction of murine intestinal and hepatic peroxiredoxin MSP23 by dietary butylated hydroxyanisole Carcinogenesis, May 1, 2000; 21(5): 1013 - 1016. [Abstract] [Full Text] [PDF]

				M. Vargas, J. G. Lamb, and M. R. Franklin Phase II-Selective Induction of Hepatic Drug-Metabolizing Enzymes by Oltipraz [5-(2-Pyrazinyl)-4-methyl-1,2-dithiol-3-thione], 1,7-Phenanthroline, and 2,2'-Dipyridyl in Rats Is Not Accompanied by Induction of Intestinal Enzymes Drug Metab. Dispos., February 1, 1998; 26(2): 91 - 97. [Abstract] [Full Text]

				R. Yu, T.-H. Tan, and A.-N. T. Kong Butylated Hydroxyanisole and Its Metabolite tert-Butylhydroquinone Differentially Regulate Mitogen-activated Protein Kinases. THE ROLE OF OXIDATIVE STRESS IN THE ACTIVATION OF MITOGEN-ACTIVATED PROTEIN KINASES BY PHENOLIC ANTIOXIDANTS J. Biol. Chem., November 14, 1997; 272(46): 28962 - 28970. [Abstract] [Full Text] [PDF]