This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Natori, T. Articles by Appella, E. Search for Related Content PubMed PubMed Citation Articles by Natori, T. Articles by Appella, E.

Immunochemical Evidence of a Tumor-specific Surface Antigen Obtained by Detergent Solubilization of the Membranes of a Chemically Induced Sarcoma, Meth-A

Laboratory of Cell Biology, National Cancer Institute, Bethesda, Maryland 20014

We reported previously the partial purification of detergent-solubilized specific tumor rejection antigen of a chemically induced sarcoma, Meth-A. During the course of the study, rabbit antiserum against a partially purified specific tumor rejection antigen preparation was raised and rendered specific by in vivo absorption. In this report we show that an antigenic molecule defined by in vivo-absorbed rabbit antiserum, which we tentatively refer to as tumor-specific surface antigen, was solubilized by detergent Nonidet P40, and extensive attempts at purification were carried out by a sequence of procedures including gel filtration, isotachophoresis, and polyacrylamide gel electrophoresis. The most highly purified tumor-specific surface antigen retained some specific tumor rejection antigen activity, suggesting an association of the two activities. Both antigens were shown to have a molecular weight of about 60,000 and an electrophoretic mobility of -globulin.

¹ Present address: Department of Pathology, Hokkaido University School of Medicine, Sapporo, 060, Japan.

² To whom requests for reprints should be addressed.

Received 8/12/77. Accepted 11/ 8/77.