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Enhancement of X-ray Transformation by 12-O-Tetradecanoyl-phorbol-13-acetate in a Cloned Line of C3H Mouse Embryo Cells¹

Laboratory of Radiobiology, Department of Physiology, Harvard School of Public Health, Boston, Massachusetts 02115 [A. R. K., J. B. L.], and Cancer Research Laboratory, Los Angeles County-University of Southern California Comprehensive Cancer Center, Los Angeles, California 90033 [S. M., C. H.]

A phorbol ester promoting agent, 12-O-tetradecanoyl-phorbol-13-acetate, enhances X-ray transformation in vitro in a two-stage fashion similar to that shown previously for ultraviolet radiation and chemical carcinogens. In studies with a mouse embryo-derived cell line (C3H/10T clone 8), there were clear interactive effects between X-radiation and 12-O-tetradecanoylphorbol-13-acetate. These were particularly marked when a minimally transforming X-ray dose (50 or 100 rads) was followed by 12-O-tetradecanoylphorbol-13-acetate treatment beginning either immediately after the radiation exposure or 48 to 96 hr later.

¹ This investigation was supported by National Cancer Institute Contracts NO1 CP 33273 and CP 65831 and by National Institute of Environmental Health Sciences Grant ES-00002.

² To whom requests for reprints should be addressed, at Laboratory of Radiobiology, Department of Physiology, Harvard School of Public Health, 665 Huntington Avenue, Boston, Mass. 02115.

Received 8/ 8/77. Accepted 11/11/77.

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