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Amino-terminal Sequence of a Carcinoembryonic Antigen-like Glycoprotein Isolated from the Colonic Lavages of Healthy Individuals¹

Division of Immunology, City of Hope National Medical Center, Duarte, California 91010 [J. E. S., C. W. T., M. L. E.], and Gastroenterology Unit, Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55901 [V. L. W. G.]

A glycoprotein has been isolated from the colonic lavages of healthy individuals that is immunologically equivalent to carcinoembryonic antigen purified from tumor tissue. The NH₂-terminal sequence of the glycoprotein from normal colon lavages is Lys-Leu-Thr-Ile-Glu-Ser-Thr-Pro-Phe-(Asn)-Val-Ala-Glu-Gly-Lys-Glu-Val-(Leu,Ile)-(Leu,Ile)-(Leu,Ile)-Val-(His,Arg?)-?-(Leu,Ile). This is homologous to the NH₂-terminal sequence of 23 of the first 24 amino acids of carcinoembryonic antigen isolated from tumor tissue.

¹ This research was supported by USPHS Grants CA 19163, CA 17491, and CA 22318 from the National Large Bowel Cancer Project and Grants CA 16434 and CB 23854, all of the National Cancer Institute.

² Present address: Department of Microbiology, University of Alabama in Birmingham, University Station, Birmingham, Ala. 35294. To whom requests for reprints should be addressed.

Received 7/25/77. Accepted 11/18/77.