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Mutagenicity of Carcinogenic Mycotoxins in Salmonella typhimurium¹

Laboratory of Microbial Chemistry and Toxicology, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Ichigaya, Shinjuku-ku, Tokyo 162, Japan

Fifteen mycotoxins and two chemically modified toxins were tested for mutagenic ability in the His⁺ revertant assay with the use of Salmonella typhimurium TA 98 and TA 100. Bisfuranoid mycotoxins such as aflatoxin B₁, aflatoxin G₁, sterigmatocystin, and O-acetylsterigmatocystin were positive in the routine test method, and epoxide mycotoxins such as PR-toxin and crotocin were positive only when the test strains were preincubated with the mycotoxins in the presence of the fortified S-9 (supernatant fraction, 9000 x g for 20 min). Anthraquinoid hepatocarcinogens such as (-)-luteoskyrin and (+)-rugulosin; lactones such as citrinin, penicillic acid, and patulin; and chlorinated carcinogens such as chloropeptide and griseofulvin were negative in the routine and preincubation assay methods. Fusarium mycotoxins such as trichothecenes and zearalenone also failed to demonstrate mutagenicity. However, among the trichothecene mycotoxins, crotocin, a bis-epoxide trichothecene, enhanced the mutagenicity of aflatoxin B₁. In vitro supplement of glutathione to the S-9 mixture enhanced the mutagenicity of aflatoxin B₁, and prior administration of various hepatotoxic agents to rats reduced the S-9-dependent mutagenicity of aflatoxin B₁. Organ and species variations of aflatoxin B₁ mutagenicity were examined and their microsomal oxygenase activities were discussed.

¹ Supported in part by a grant-in-aid from the Ministry of Welfare for 1976.

Received 6/ 2/77. Accepted 11/23/77.

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