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Equivalent Expression of Endogenous Murine Leukemia Virus-related Genes in C3H/10T Cells and Chemically Transformed Derivative Cells¹

Department of Pathology and Anatomy, Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55901

The possibility that chemical carcinogens may induce enhanced expression of endogenous C-type RNA tumor virus genes in the absence of intact virus particle production has been partially tested in a model system. This was accomplished by measuring the abundance and diversity of murine leukemia virus-related RNA sequences associated with the polyribosome fraction of nontransformed C3H/10T clone 8 cells and a 3-methylcholanthrene-transformed derivative clone. Although both clones are virus nonproducers, they were found to contain significant amounts of polyadenylate-containing murine leukemia virus-related RNA sequences; however, both the types and quantities of such sequences appear indistinguishable in both clones. These results suggest that expression of the corresponding gene sequences into RNA is not related to the maintenance of the transformed state in these chemically transformed cells.

¹ This work was supported by Grant NP-192 from the American Cancer Society, by Grant CA 16816 from the National Cancer Institute, Department of Health, Education, and Welfare, and by the Mayo Foundation.

² To whom requests for reprints should be addressed.

Received 8/26/77. Accepted 11/30/77.