This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Rudman, D. Articles by Shah, V. R. Search for Related Content PubMed PubMed Citation Articles by Rudman, D. Articles by Shah, V. R.

Isolation of Novel Glycoprotein HNC1ß from the Urine of a Patient with Acute Monocytic Leukemia¹

Department of Medicine, Emory University School of Medicine, and Clinical Research Facility, Emory University Hospital, Atlanta, Georgia 30322

Patient H. N. with acute monocytic leukemia excreted 150 to 300 mg of urinary protein per day; gel filtration showed 70% to be in the M. W. range of 20,000 to 40,000. By repeated gel filtration, and cation- and anion-exchange chromatography, the major urinary protein, labeled HNC1ß, was isolated in a yield of 10 to 30 mg/liter of urine. HNC1ß did not react with antisera to any of the recognized normal plasma proteins or to previously isolated cancer-related urinary proteins. HNC1ß contains 40% carbohydrate and 60% protein; molecular weight is 33,000. Amino acid compositions of HNC1ß and EDC1, a glycoprotein previously isolated from the urine of a patient with acute myelocytic leukemia, are closely similar, while the carbohydrate compositions differ widely.

An antiserum to HNC1ß was raised in the rabbit and a radioimmunoassay capable of detecting 10 ng of the glycoprotein was developed. Normal and cancer plasma contained similar amounts of an unidentified factor, about 150,000 in molecular weight, which reacts in the radioimmunoassay. Urine from normal individuals and 9 nonneoplastic diseases contained <0.1 mg of HNC1ß per g of creatinine. Urine from patients with 11 types of disseminated cancer (ovary, breast, lung, colon, squamous head and neck, melanoma, acute and chronic leukemias of myelocytic, monocytic and lymphocytic varieties) contained 1.7 to 27 mg of HNC1ß per g of creatinine.

¹ Supported by USPHS Grants CA12646, RR00039, and CA20997.

² To whom requests for reprints should be addressed.

Received 8/12/77. Accepted 11/23/77.