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Chromatin-asscoiated Glycoproteins of Normal Rat Liver and Novikoff Hepatoma Ascites Cells¹

Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030

Glycoproteins were demonstrated in the 0.6 M NaCl extract of chromatin of normal liver and Novikoff hepatoma cells by periodic acid-Schiff staining of sodium dodecyl sulfate-polyacrylamide gels. In chromatin extracts of Novikoff hepatoma cells, six major periodic acid-Schiff-positive bands coincident with Coomassie Bluestained protein bands were found with molecular weights of 30,000, 54,000, 64,000, 75,000, 104,000, and 127,000. A corresponding extract from normal rat liver chromatin revealed the presence of four major periodic acid-Schiffpositive bands that migrated with protein bands at molecular weights of 16,000, 30,000, 54,000, and 75,000.

The glycoproteins of these extracts contained either mannose or -D-glucose, fucose, and N-acetylglucosamine as shown by the specificity of lectins in affinoelectrophoresis. One of the glycoproteins detected by affinoelectrophoresis was very basic.

¹ This investigation was supported by Grant CA-10893 P.6, awarded by the National Cancer Institute, Department of Health, Education, and Welfare; the Davidson Fund; the Wolff Memorial Foundation; and a generous gift from Mrs. Jack Hutchins.

Received 10/ 7/77. Accepted 1/ 6/78.