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An Epithelial Cell Line with Chronic Polyoma Infection Established from a Spontaneous Mouse Pancreatic Adenocarcinoma¹

The Jackson Laboratory,⁴ Bar Harbor, Maine 04609

The establishment of an epithelial cell line from a mouse pancreatic adenocarcinoma is described. The cell line, designated LTPA, was aneuploid and exhibited many transformed growth properties (rapid growth rate, failure to show density-dependent inhibition of growth, ability to grow in defined medium). A type C oncornavirus was isolated from the culture medium, and electron microscopy also revealed the presence of intracisternal type A particles. LTPA cells carried a persistent polyoma infection which produced only low levels of cytopathic effects. A mycoplasmal contamination was also carried. When injected s.c. into Swiss nu/nu mice, LTPA cells formed ductular structures which were destroyed by inflammatory reactions within 3 weeks.

¹ Supported by Grant AM 17631 from the National Institute of Arthritis, Metabolism, and Digestive Diseases.

² To whom requests for reprints should be addressed.

³ Participant in the 1976 Summer Program for College Students at The Jackson Laboratory.

⁴ The Jackson Laboratory is fully accredited by The American Association for Accreditation of Laboratory Animal Care.

Received 8/26/77. Accepted 1/ 3/78.