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Identification of Neoantigenic Tumor Surface Glycoproteins in Non-ionic Detergent Extracts of Rat Rous Sarcomas¹

Lady Davis Institute for Medical Research of the Jewish General Hospital, Montreal, Quebec, Canada H3T 1E2

Cultured rat cells, derived from Rous sarcoma virus-induced tumors or from rat embryos, were radiolabeled by incorporation of L-[6-³H]fucose or by lactoperoxidasecatalyzed iodination of cell surface proteins. Non-ionic detergent extracts of radiolabeled cells were prepared, incubated with "nonimmune immunoglobulin G (IgG)" (lacking antitumor antibody) or "immune IgG" [produced in a W/Fu rat following inoculation with W/Fu rat sarcoma cells transformed by subgroup D, Schmidt-Ruppin strain (SRV-D) of Rous sarcoma virus], and putative soluble immune complexes were precipitated with antirat IgG. Immune precipitates were analyzed for specific radiolabeled antigen by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Immune IgG reacted with two radiolabeled glycoproteins in the extracts of SRV-D-induced W/Fu rat Rous sarcoma lines but displayed no activity against Rous sarcoma cells that were derived from rats of unspecified genetic strain and that had been induced by Rous sarcoma virus of strains other than the SRV-D. One of these glycoproteins migrated at a position of an apparent molecular weight of approximately 60,000 daltons, and the other did so at about 35,000 daltons. These experiments demonstrate the existence of neoantigen at the surfaces of rat Rous sarcoma cells. Whether these antigens are absolutely tumor specific is not known, but it is apparent that the specificity of the determinants is stringently restricted by host or virus genetic strain.

¹ This research was supported by grants from the Cancer Research Society, Inc., Montreal, Quebec, Canada, and the National Cancer Institute of Canada.

² To whom requests for reprints should be addressed, at Lady Davis Institute for Medical Research of the Jewish General Hospital, 3755 Cote St. Catherine Road, Montreal, Quebec, Canada H3T 1E2.

Received 8/30/77. Accepted 1/31/78.