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Differences in Total Mitochondrial Proteins and Proteins Synthesized by Mitochondria from Rat Liver and Morris Hepatomas 9618A, 5123C, and 5123tc¹

Department of Biochemistry, Wayne State University School of Medicine, Detroit, Michigan 48201 [C. C. I., L. I. M.], and Department of Biochemistry, Howard University School of Medicine, Washington, D. C. 20001 [H. P. M.]

Mitochondria were isolated from a slow-growing (9618A) and two intermediate-to-fast-growing (5123C, 5123tc) Morris hepatomas and host livers. The mitochondrial proteins were solubilized and fractionated on sodium dodecyl sulfate:polyacrylamide slab gels. One Coomassie bluestained band was absent or reduced in amount in all tumors relative to host livers. In addition, a major mitochondrial enzyme present in normal liver, carbamyl phosphate synthetase, was missing or greatly reduced in the slow-growing, highly differentiated hepatoma 9618A, a tumor that is considered to be similar to normal liver in many biochemical and morphological respects. Incubation of mitochondria with [³⁵S]methionine and a suitable amino acid incorporation system resulted in labeling of specific mitochondrial proteins. Autoradiography of the slab gels disclosed four prominently labeled fractions and a number of minor fractions. Preparations from hepatoma 5123tc demonstrated two labeled bands that were absent or greatly reduced in host liver. Host liver preparations displayed a minor band that was absent or greatly reduced in hepatoma 5123C. However, no single change in labeling pattern was common to all three tumors, suggesting the absence of a causal relationship between carcinogenesis and mutations in mitochondrial DNA.

¹ Supported by the National Cancer Institute (CA 15539), the National Science Foundation (BMS73-02031), the Research Corporation (BH512), and in part by USPHS Grant CA 10729.

² Fellow of the National Cancer Institute (CA 05109). Present address: Eunice Kennedy Shriver Center for Mental Retardation, Waltham, Mass. 02154.

³ To whom requests for reprints should be addressed.

Received 8/26/77. Accepted 3/ 7/78.

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