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Effects of Confluent Monolayers of Density-inhibited and -transformed Cells on the Growth of Superinoculated Cells¹

Divisions of Physiology and Pathology [H. S., T. T.] and Genetics [K. S.], National Institute of Radiological Sciences, 4-9-1, Anagawa, Chiba-shi, Chiba, Japan

The effect of confluent monolayers of density-inhibited (A31-714, 10T, and Hepano) and transformed (SEA-7, HSV-2-5, and HT2B-1) cells on the proliferation of superinoculated cells was examined. Most monolayers were lethally irradiated. The growth of density-inhibited cells was inhibited when they were inoculated on a monolayer of homologous cells. In contrast the growth pattern of transformed cells on a homologous cell sheet was not different from that on an empty plate. The growth capacity of density-inhibited cells on a monolayer of heterologous density-inhibited cells was as follows: (a) the growth of A31-714 cells was inhibited by neither 10T nor Hepano cells; (b) 10T cells proliferated well on Hepano cells but not on A31-714 cells; (c) the growth of Hepano cells was inhibited by both A31-714 and 10T cells; (d) densityinhibited cells were able to proliferate on top of homologous cells once the basal cell layer was fixed with either glutaraldehyde or osmic acid; (e) the effect of a monolayer of two types of transformed cells on superinoculated density-inhibited cells was also tested. HSV-2-5 cells restricted the growth of density-inhibited cells, but SEA-7 cells did not.

These growth-regulatory effects do not seem to be mediated by soluble substances released into the culture medium by cells.

¹ Present studies were supported by Grant 101051 for Cancer Research from the Japanese Ministry of Education, Science, and Culture.

Received 3/ 6/78. Accepted 6/ 1/78.