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Comparison of Four New Cell Lines from Patients with Adenocarcinoma of the Ovary¹

Department of Surgery, Division of Surgical Oncology, Denver General Hospital, Denver, Colorado 80204

Permanent human tumor cell lines COLO 110, COLO 316, COLO 319, and COLO 330 were established from four patients with serous cystadenocarcinoma of the ovary. COLO 110 was derived from primary tumor tissue; COLO 316, COLO 319, and COLO 330 were derived from cells in malignant effusions. COLO 110 and COLO 316 grew as monolayers of epithelioid cells in culture; COLO 319 and COLO 330 grew as vermiform, floating colonies of epithelioid cells in culture. Epithelial-like morphology was confirmed by transmission electron microscopy. All four cell lines had marker chromosomes and double minute chromosomes. Giemsa banding revealed chromosomes 1, 3, 6, and 7 were involved in markers in all four lines, and chromosomes 2, 4, 5, 9, 11, and 15 were involved in markers in three of the cell lines. Marker chromosomes with possible homogeneous staining regions were observed in COLO 319. Estrone was elaborated by three of the lines, but neither chorionic gonadotropin, carcinoembryonic antigen, nor estrogen or progesterone receptor proteins were detected. Each cell line demonstrated a distinctive isozyme phenotype. These cell lines are maintained in active culture and in a cell bank for distribution to other investigators.

¹ This investigation was supported by Grant 1-R01-CA15018, awarded by the National Cancer Institute, Department of Health, Education, and Welfare and by the Mary B. and L. H. Marshall Fund.

² To whom requests for reprints should be addressed, at Department of Surgery, Division of Surgical Oncology, Denver General Hospital, West Eighth Avenue & Cherokee Street; Denver, Colo. 80204.

Received 5/ 1/79. Accepted 8/ 7/79.