This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tsuda, H. Articles by Bueding, E. Search for Related Content PubMed PubMed Citation Articles by Tsuda, H. Articles by Bueding, E.

Induction of Hepatic Neoplastic Lesions in Mice with a Single Dose of Hycanthone Methanesulfonate after Partial Hepatectomy¹

Fels Research Institute, Temple University School of Medicine, Philadelphia, Pennsylvania 19140 [D. S. R. S., S. R., J. Z., E. F.]; Department of Pathology, University of Toronto, Medical Sciences Building, Toronto, Ontario, Canada, M5S 1A8 [H. T., D. S. R. S., S. R., E. F.]; and Department of Pathobiology, School of Hygiene and Public Health, the Johns Hopkins University, Baltimore, Maryland 21205 [R. P. B., Y-N. C., E. B.]

Experiments were designed to determine whether hycanthone methanesulfonate (1-{[2-(diethylamino)ethyl]amino}-4-(hydroxymethyl)thioxanthen-9-one monomethanesulfonate), an antischistosomal drug, and its analog, IA-4-N-oxide (8-chloro-2-[2-(diethylamino)ethyl]-2H-[1]benzothiopyrano[4,3,2-cd]indazole 5-methanol monomethanesulfonate), will induce neoplastic lesions in the livers of mice not infected with Schistosoma mansoni. All the mice received a single i.m. injection of hycanthone methanesulfonate (76 mg/kg), IA-4-N-oxide (80 mg/kg), or an equivalent volume of the solvent, 0.9% NaCl solution, 42 hr after partial hepatectomy. Of the mice receiving hycanthone methanesulfonate and living 200 days or longer, hepatocellular carcinoma was seen in 11.5% and liver sarcoma was seen in 4.2%. This type of malignant neoplasm was not seen in the animals receiving either IA-4-N-oxide or 0.9% NaCl solution. In addition, mice receiving hycanthone methanesulfonate showed a significantly higher incidence of both type 1 (43% compared to 21% in controls) and type 2 (21% compared to 12% in controls) hepatocyte neoplasms. Mice receiving IA-4-N-oxide showed no increased incidence of neoplasms.

¹ This investigation was supported in part by USPHS Grants CA-14689, CA-12218, CA-12227, CA-18251, and GM-16492 from the NIH and by a grant from the National Cancer Institute of Canada.

² To whom requests for reprints should be addressed, at Department of Pathology, University of Toronto, Medical Sciences Building, 1 Kings College Circle, Toronto, Ontario, Canada, M5S 1A8.

Received 2/ 5/79. Accepted 8/10/79.

This article has been cited by other articles:

				B. Ames, R Magaw, and L. Gold Ranking possible carcinogenic hazards Science, April 17, 1987; 236(4799): 271 - 280. [Abstract] [PDF]