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Adaptation of Sinclair Swine Melanoma Cells to Long-Term Growth in Vitro¹

Cancer Research Center [J. B., S. M. C., E. H. A., R. W. O., R. R. H., Jr.], Sinclair Comparative Medicine Research Farm [J. B., R. R. H., Jr.], Department of Pathology, Ellis Fischel State Cancer Hospital [R. W. O.], Department of Medicine [J. B.], Pathology [E. H. A., R. W. O.], and Microbiology [R. R. H., Jr.] and Division of Biological Sciences [J. B.], University of Missouri, Columbia, Missouri 65201, and Department of Medicine, Roger Williams General Hospital, Providence 02908 [D. L. D.], and Section of Medicine, Brown University, Providence, Rhode Island 02912 [D. L. D.]

Sinclair swine melanoma usually regresses in vivo. In the present study, swine melanoma cells were adapted to long-term growth in culture. The morphology of cultured melanoma cells ranged from dendritic to cuboidal, similar to that described for human melanoma cells. Doubling times of the swine melanoma cells were also similar to those of human melanoma cells in vitro. 3,4-Dihydroxy-L-phenylalanine oxidase-positive cells were detected by light microscopy, and melanin and premelanosomes were detected by electron microscopy. Cell cultures could be propagated from progressing, partially regressed, and primary cutaneous lesions, as well as from visceral metastases. Thus, it appears that, under these cell culture conditions, Sinclair swine melanoma cells can be adapted to prolonged growth in vitro.

¹ This investigation supported by Grants CA 08023, CA 19409, and CA 25718 from the National Cancer Institute, Department of Health, Education, and Welfare.

² To whom requests for reprints should be addressed, at Sinclair Comparative Medicine Research Farm, Route 3, Columbia, Mo. 65201.

Received 5/29/79. Accepted 9/11/79.