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Presence and Cell Growth-related Variations of Glycogen in Human Colorectal Adenocarcinoma Cell Lines in Culture¹

Groupe de Recherches sur l'Immunologie de la Différenciation (INSERM U178), Hópital Broussais 96 rue Didot, 75674 Paris Cedex 14, France [M. R., G. C., E. D., A. Z.], and Institut de Recherches en Biologie Moléculaire, Université Paris VII, Tour 43, 2 Place Jussieu, 75221 Paris Cedex 05, France [J.-P. R.]

The presence and kinetics of intracellular glycogen levels were studied, in relationship to cell growth, in asynchronous and in synchronized cultures of four human colorectal adenocarcinoma cell lines (HT-29, HRT-18, SW-480, and Caco-2). The results show that a specific pattern of glycogen accumulation occurs during the process of cell growth of the studied cell lines. The kinetics of glycogen accumulation in asynchronous cultures were similar from one cell line to another and were characterized by a low amount in the exponential phase of growth, followed by a 3- to 4-fold increase in the stationary phase. The quantities found in either phase were specific for each cell line. The maximum values found in Caco-2, HRT-18, HT-29, and SW-480 cells were, respectively, 258.5 ± 6.9 (S.D.), 88.9 ± 2.6, 87.5 ± 3, and 17.5 ± 1.8 µg of glycogen per mg of proteins. The kinetics of glycogen accumulation during the cell cycle was also studied in synchronized cultures of HT-29 and HRT-18 cell lines. Both cell lines exhibited a common pattern of low glycogen quantities during S, G₂, and M followed by an increase beginning with G₁ and peaking (2.5 to 3 times the initial values) in the middle of this phase. This was followed by a symmetrical decrease in the second half of G₁.

¹ Supported by Institut National de la Sante et de la Recherche Medicale, CRL 77.5.153.1.

² To whom requests for reprints should be addressed.

Received 6/ 5/78. Accepted 10/27/78.

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